What temp to pour agar Once the bottle of media is cool enough (to about 45–55° C), the agar is ready to pour. Inoculation of agar plates with sterile swabs from environmental surfaces 4. One of the classic uses of agar is for the preparation of microbial plates where the combined properties of low syneresis, no dependence on ions and low set temperature make agar ideal. Science Stuff, Inc. ) 2. 128 132. 5% w/v), and cover it with foil and a small piece of autoclave tape. Avans Biotechnology Corp. You could also make multiples of 200 ml in 250 ml bottles, but don’t make >400 ml in a 1 L bottle – this is too heavy to easily pour plates with. Store plates: Once set, plates can be stored upside Give 2 reasons why the nutrient agar must be cooled to 45°C before inoculating and pouring: Lower temperature results in less condensation. cqmhageqfxcfufgdqcqxsvenchgkeuqctwgcoxjdflgcruttvzhmrxdoaasxvktyrcsyizi